{"id":6400,"date":"2023-05-14T02:18:17","date_gmt":"2023-05-14T02:18:17","guid":{"rendered":"http:\/\/sunjin.vn\/tin-tuc-thuy-san-so-e02-022023\/"},"modified":"2023-05-14T02:18:17","modified_gmt":"2023-05-14T02:18:17","slug":"tin-tuc-thuy-san-so-e02-022023","status":"publish","type":"post","link":"http:\/\/sunjin.vn\/en\/tin-tuc-thuy-san-so-e02-022023\/","title":{"rendered":"Tin t\u1ee9c Th\u1ee7y s\u1ea3n s\u1ed1 E02- 02\/2023"},"content":{"rendered":"<div class=\"editer\">\n<p>Sunjin \u0111\u1ea7u t\u01b0 x\u00e2y d\u1ef1ng nh\u00e0 m\u00e1y Th\u1ee7y s\u1ea3n t\u1ea1i t\u1ec9nh H\u1eadu Giang &amp; chuy\u00ean m\u1ee5c k\u1ef9 thu\u1eadt &#8211; T\u00ecm hi\u1ec3u v\u1ec1 Wenzhou virus 8 (WZV8) tr\u00ean t\u00f4m v\u00e0 ph\u01b0\u01a1ng ph\u00e1p ph\u00e1t hi\u1ec7n<\/p>\n<p style=\"text-align: center;\" align=\"center\"><strong><span style=\"font-family: times new roman,serif;\"><span style=\"font-size: 22.0pt;\">TIN T\u1ee8C <span style=\"color: red;\">SUNJIN<\/span><\/span><\/span><\/strong><\/p>\n<p style=\"text-align: right;\" align=\"right\"><strong><span style=\"font-family: times new roman,serif;\"><span style=\"font-size: 13.0pt;\">S\u1ed1 E02 \u2013 02\/2023<\/span><\/span><\/strong><\/p>\n<p style=\"text-align: justify; text-justify: inter-ideograph;\"><strong><span style=\"font-family: times new roman,serif;\"><span style=\"font-size: 13.0pt;\">Sunjin \u0111\u1ea7u t\u01b0 x\u00e2y d\u1ef1ng nh\u00e0 m\u00e1y Th\u1ee7y s\u1ea3n t\u1ea1i t\u1ec9nh H\u1eadu Giang<\/span><\/span><\/strong><\/p>\n<p style=\"text-align: justify; text-justify: inter-ideograph;\"><span style=\"font-family: times new roman,serif;\"><span style=\"font-size: 13.0pt;\">\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Ngay ng\u00e0y l\u00e0m vi\u1ec7c \u0111\u1ea7u ti\u00ean sau k\u1ef3 ngh\u1ec9 T\u1ebft Nguy\u00ean \u0111\u00e1n Qu\u00fd M\u00e3o 2023 (27\/1), Sunjin \u0111\u00e3 \u0111\u01b0\u1ee3c Ban Qu\u1ea3n l\u00fd c\u00e1c khu c\u00f4ng nghi\u1ec7p (QLCKCN) t\u1ec9nh H\u1eadu Giang trao gi\u1ea5y ch\u1ee9ng nh\u1eadn \u0111\u0103ng k\u00fd \u0111\u1ea7u t\u01b0 D\u1ef1 \u00e1n Nh\u00e0 m\u00e1y s\u1ea3n xu\u1ea5t th\u1ee9c \u0103n Th\u1ee7y s\u1ea3n Sunjin sau khi \u0111\u00e3 ti\u1ebfn h\u00e0nh ph\u00e2n t\u00edch, ki\u1ec3m tra v\u00e0 th\u0103m quan nh\u00e0 m\u00e1y hi\u1ec7n t\u1ea1i c\u1ee7a Sunjin.<\/span><\/span><\/p>\n<img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-3541 size-full\" src=\"http:\/\/sunjin.vn\/wp-content\/uploads\/2023\/05\/E02_1.png\" alt=\"\" width=\"1199\" height=\"336\" \/>\n<p style=\"text-align: center;\" align=\"center\"><strong><em><span style=\"font-family: times new roman,serif;\">Bu\u1ed5i th\u0103m quan c\u00f4ng ty Sunjin c\u1ee7a \u0111o\u00e0n \u0110\u1ea1i bi\u1ec3u t\u1ec9nh H\u1eadu Giang v\u00e0o th\u00e1ng 9\/2022<\/span><\/em><\/strong><\/p>\n<p style=\"text-align: justify; text-justify: inter-ideograph;\"><span style=\"font-family: times new roman,serif;\"><span style=\"font-size: 13.0pt;\">\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 D\u1ef1 \u00e1n do C\u00f4ng ty TNHH Sunjin Vina l\u00e0m ch\u1ee7 \u0111\u1ea7u t\u01b0, \u0111\u1eb7t t\u1ea1i Khu c\u00f4ng nghi\u1ec7p T\u00e2n Ph\u00fa Th\u1ea1nh, huy\u1ec7n Ch\u00e2u Th\u00e0nh A; v\u1edbi t\u1ed5ng di\u1ec7n t\u00edch 2,6 ha; t\u1ed5ng v\u1ed1n \u0111\u1ea7u t\u01b0 23,6 tri\u1ec7u USD (t\u01b0\u01a1ng \u0111\u01b0\u01a1ng h\u01a1n 553 t\u1ef7 \u0111\u1ed3ng). \u0110\u00e2y l\u00e0 d\u1ef1 \u00e1n s\u1ea3n xu\u1ea5t s\u1ea3n ph\u1ea9m th\u1ee9c \u0103n Th\u1ee7y s\u1ea3n (c\u00e1, t\u00f4m), s\u1eed d\u1ee5ng c\u00f4ng ngh\u1ec7 ti\u00ean ti\u1ebfn, quy tr\u00ecnh s\u1ea3n xu\u1ea5t kh\u00e9p k\u00edn, c\u00f4ng su\u1ea5t 126.000 t\u1ea5n\/n\u0103m. D\u1ef1 ki\u1ebfn khi \u0111i v\u00e0o ho\u1ea1t \u0111\u1ed9ng, doanh thu m\u1ed7i n\u0103m c\u1ee7a D\u1ef1 \u00e1n \u0111\u1ea1t 161,4 tri\u1ec7u USD, gi\u00e1 tr\u1ecb xu\u1ea5t kh\u1ea9u \u0111\u1ea1t 40 tri\u1ec7u USD. Theo k\u1ebf ho\u1ea1ch, giai \u0111o\u1ea1n 1 c\u1ee7a D\u1ef1 \u00e1n s\u1ebd ch\u00ednh th\u1ee9c \u0111i v\u00e0o ho\u1ea1t \u0111\u1ed9ng trong th\u00e1ng 5\/2024.<\/span><\/span><\/p>\n<img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-3540 size-full\" src=\"http:\/\/sunjin.vn\/wp-content\/uploads\/2023\/05\/E02_2.png\" alt=\"\" width=\"1176\" height=\"398\" \/>\n<p style=\"text-align: center;\" align=\"center\"><strong><em><span style=\"font-family: times new roman,serif;\">L\u1ec5 trao gi\u1ea5y ch\u1ee9ng nh\u1eadn \u0111\u0103ng k\u00fd \u0111\u1ea7u t\u01b0 D\u1ef1 \u00e1n Nh\u00e0 m\u00e1y s\u1ea3n xu\u1ea5t th\u1ee9c \u0103n th\u1ee7y s\u1ea3n Sunjin<\/span><\/em><\/strong><\/p>\n<p style=\"text-align: justify; text-justify: inter-ideograph;\"><span style=\"font-family: times new roman,serif;\"><span style=\"font-size: 13.0pt;\">\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Sunjin g\u1eedi l\u1eddi c\u1ea3m \u01a1n ch\u00e2n th\u00e0nh \u0111\u1ebfn Ban Qu\u1ea3n l\u00fd c\u00e1c khu c\u00f4ng nghi\u1ec7p (QLCKCN) t\u1ec9nh H\u1eadu Giang \u0111\u00e3 xem x\u00e9t v\u00e0 th\u00f4ng qua d\u1ef1 \u00e1n \u0111\u1ea7u t\u01b0 nh\u00e0 m\u00e1y c\u1ee7a ch\u00fang t\u00f4i. Sunjin s\u1ebd c\u1ed1 g\u1eafng nh\u1ea5t c\u00f3 th\u1ec3 \u0111\u1ec3 ho\u00e0n th\u00e0nh d\u1ef1 \u00e1n \u0111\u00fang ti\u1ebfn \u0111\u1ed9 v\u00e0 \u0111\u01b0a v\u00e0o ho\u1ea1t \u0111\u1ed9ng nh\u01b0 k\u1ebf ho\u1ea1ch \u0111\u00e3 \u0111\u1ec1 ra.<\/span><\/span><\/p>\n<p style=\"text-align: justify; text-justify: inter-ideograph;\"><strong><span style=\"font-family: times new roman,serif;\"><span style=\"font-size: 13.0pt;\">Chuy\u00ean m\u1ee5c k\u1ef9 thu\u1eadt &#8211; T\u00ecm hi\u1ec3u v\u1ec1 Wenzhou virus 8 (WZV8) tr\u00ean t\u00f4m v\u00e0 ph\u01b0\u01a1ng ph\u00e1p ph\u00e1t hi\u1ec7n<\/span><\/span><\/strong><\/p>\n<p style=\"margin-bottom: 6.0pt; text-align: justify; text-justify: inter-ideograph; text-indent: 36.0pt;\"><span style=\"font-family: times new roman,serif;\"><span style=\"font-size: 13.0pt;\">Ng\u00e0nh t\u00f4m lu\u00f4n \u0111\u01b0\u1ee3c coi l\u00e0 ng\u00e0nh kinh t\u1ebf m\u0169i nh\u1ecdn c\u1ee7a nhi\u1ec1u qu\u1ed1c gia c\u00f3 l\u1ee3i th\u1ebf \u0111\u1ecba l\u00fd gi\u00e1p bi\u1ec3n ph\u1ea3i k\u1ec3 \u0111\u1ebfn nh\u01b0 Vi\u1ec7t Nam, Trung Qu\u1ed1c, Th\u00e1i Lan,&#8230; C\u00f9ng v\u1edbi s\u1ef1 quan t\u00e2m d\u00e0nh cho ng\u00e0nh t\u00f4m, vi\u1ec7c nghi\u00ean c\u1ee9u v\u1ec1 b\u1ec7nh h\u1ecdc tr\u00ean t\u00f4m ng\u00e0nh c\u00e0ng \u0111\u01b0\u1ee3c ch\u00fa tr\u1ecdng \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o m\u1ed9t v\u1ee5 m\u00f9a thu\u1eadn l\u1eddi cho ng\u01b0\u1eddi nu\u00f4i.<\/span><\/span><\/p>\n<p style=\"margin-bottom: 6.0pt; text-align: justify; text-justify: inter-ideograph; text-indent: 36.0pt;\"><span style=\"font-family: times new roman,serif;\"><span style=\"font-size: 13.0pt;\">Ng\u00e0y nay c\u00f9ng v\u1edbi s\u1ef1 ph\u00e1t tri\u1ec3n c\u1ee7a khoa h\u1ecdc &#8211; c\u00f4ng ngh\u1ec7, c\u00e1c nh\u00e0 nghi\u00ean c\u1ee9u Th\u00e1i Lan \u0111\u00e3 ph\u00e1t hi\u1ec7n s\u1ef1 c\u00f3 m\u1eb7t c\u1ee7a m\u1ed9t lo\u00e0i vi r\u00fat mang t\u00ean <em>Wenzhou virus 8<\/em> (WZV8) xu\u1ea5t hi\u1ec7n trong c\u1ea3 nh\u1eefng m\u1eabu t\u00f4m th\u01b0\u1eddng v\u00e0 t\u00f4m s\u1eafp ch\u1ebft thu t\u1ea1i c\u00e1c v\u00f9ng Ch\u00e2u \u00c1- \u0110\u1ea1i T\u00e2y D\u01b0\u01a1ng v\u00e0 hai qu\u1ed1c gia \u1edf Ch\u00e2u M\u1ef9 b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p d\u00f9ng k\u00ednh hi\u1ec3n vi \u0111i\u1ec7n t\u1eed truy\u1ec1n qua (TEM) v\u00e0 ph\u01b0\u01a1ng ph\u00e1p x\u00e9t nghi\u1ec7m ph\u00e2n t\u1eed. <\/span><\/span><\/p>\n<p style=\"margin-bottom: 6.0pt; text-align: justify; text-justify: inter-ideograph; text-indent: 36.0pt;\"><span style=\"font-family: times new roman,serif;\"><span style=\"font-size: 13.0pt;\">K\u1ebft lu\u1eadn sau khi nghi\u00ean c\u1ee9u cho th\u1ea5y, \u0111\u1ebfn nay ch\u01b0a c\u00f3 b\u1eb1ng ch\u1ee9ng n\u00e0o ch\u1ec9 ra c\u00e1c virus WZV8 \u0111\u00e3 g\u00e2y b\u1ec7nh. Tuy nhi\u00ean, c\u00f3 th\u1ec3 t\u1ed3n t\u1ea1i c\u00e1c lo\u1ea1i WZV8 kh\u00e1c v\u00e0 m\u1ed9t s\u1ed1 lo\u1ea1i \u0111\u00f3 c\u00f3 th\u1ec3 g\u00f3p ph\u1ea7n g\u00e2y t\u1eed vong khi k\u1ebft h\u1ee3p v\u1edbi m\u1ed9t ho\u1eb7c nhi\u1ec1u m\u1ea7m b\u1ec7nh kh\u00e1c ho\u1eb7c trong m\u1ed9t s\u1ed1 \u0111i\u1ec1u ki\u1ec7n m\u00f4i tr\u01b0\u1eddng.<\/span><\/span><\/p>\n<ul>\n<li style=\"margin-bottom: 6pt; text-align: justify;\"><strong><em><u><span style=\"font-family: times new roman,serif;\"><span style=\"font-size: 13.0pt;\">Ph\u00e1t hi\u1ec7n WZV8 b\u1eb1ng quan s\u00e1t th\u1ec3 v\u00f9i<\/span><\/span><\/u><\/em><\/strong><\/li>\n<\/ul>\n<p style=\"margin-bottom: 6.0pt; text-align: justify; text-justify: inter-ideograph; text-indent: 36.0pt;\"><span style=\"font-family: times new roman,serif;\"><span style=\"font-size: 13.0pt;\">Theo c\u00e1c nh\u00e0 nguy\u00ean c\u1ee9u Th\u00e1i Lan, virus WZV8 thu\u1ed9c b\u1ed9\u00a0<em>Picornavirales<\/em> (s\u1ed1 truy c\u1eadp Ng\u00e2n h\u00e0ng gen KX883984) xu\u1ea5t hi\u1ec7n d\u01b0\u1edbi d\u1ea1ng c\u00e1c th\u1ec3 v\u00f9i, c\u00f3 m\u1eb7t c\u1eaft h\u00ecnh tr\u00f2n, \u0111\u01b0\u1ee3c ch\u1ee9a trong kh\u00f4ng b\u00e0o c\u1ee7a t\u1ebf b\u00e0o ch\u1ea5t ho\u1eb7c trong bi\u1ec3u m\u00f4 \u1ed1ng h\u1ec7 gan t\u1ee5y c\u1ee7a t\u00f4m. \u0110\u1ec3 quan s\u00e1t c\u00e1c th\u1ec3 v\u00f9i WZV5 r\u00f5 h\u01a1n, c\u00e1c nh\u00e0 nghi\u00ean c\u1ee9u s\u1ebd nhu\u1ed9m ch\u00fang v\u1edbi c\u00e1c thu\u1ed1c nhu\u1ed9m nh\u01b0 <em>hematoxylin &amp; eosin<\/em> (H&amp;E) ho\u1eb7c <em>toluidine<\/em>, t\u1eeb \u0111\u00f3 s\u1ebd c\u00f3 c\u00e1c m\u00e0u s\u1eafc kh\u00e1c nhau nh\u01b0 t\u00edm ho\u1eb7c xanh lam \u0111\u1eb7c tr\u01b0ng cho t\u1eebng ph\u01b0\u01a1ng ph\u00e1p nhu\u1ed9m.<\/span><\/span><\/p>\n<img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-3539 size-full\" src=\"http:\/\/sunjin.vn\/wp-content\/uploads\/2023\/05\/E02_3.png\" alt=\"\" width=\"1135\" height=\"555\" \/>\n<p style=\"margin-bottom: .0001pt; text-align: center;\" align=\"center\"><strong><em><span style=\"font-family: times new roman,serif;\">C\u00e1c th\u1ec3 v\u00f9i WZV8 ch\u1ee5p \u0111\u01b0\u1ee3c trong kh\u00f4ng b\u00e0o t\u1ebf b\u00e0o ph\u00f4i<\/span><\/em><\/strong><\/p>\n<p style=\"margin-bottom: .0001pt; text-align: justify; text-justify: inter-ideograph;\"><em><span style=\"font-family: times new roman,serif;\">A: H\u00ecnh ch\u1ee5p vi m\u00f4 c\u1ee7a th\u1ec3 v\u00f9i WZV8 ph\u1ed5 bi\u1ebfn nh\u1ea5t, h\u00ecnh tr\u00f2n trong kh\u00f4ng b\u00e0o c\u1ee7a t\u1ebf b\u00e0o ph\u00f4i<\/span><\/em><\/p>\n<p style=\"margin-bottom: .0001pt; text-align: justify; text-justify: inter-ideograph;\"><em><span style=\"font-family: times new roman,serif;\">B: C\u00e1c bi\u1ebfn th\u1ec3 trong th\u1ec3 v\u00f9i WZV8 \u00e1i ki\u1ec1m \u0111\u00f4i, \u0111i k\u00e8m v\u1edbi nh\u1eefng th\u1ec3 v\u00f9i \u00e1i toan v\u1ec7 tinh h\u00ecnh tr\u00f2n. \u0110\u00e2y l\u00e0 m\u1ed9t s\u1ef1 k\u1ebft h\u1ee3p r\u1ea5t \u0111\u1eb7c bi\u1ec7t, c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c coi l\u00e0 b\u1ec7nh l\u00fd \u0111\u1ed1i v\u1edbi WZV8.<\/span><\/em><\/p>\n<img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-3538 size-full\" src=\"http:\/\/sunjin.vn\/wp-content\/uploads\/2023\/05\/E02_4.png\" alt=\"\" width=\"1131\" height=\"416\" \/>\n<p style=\"margin-bottom: .0001pt; text-align: center; line-height: normal;\" align=\"center\"><strong><em><span style=\"font-family: times new roman,serif;\">C\u00e1c th\u1ec3 v\u00f9i WZV8 ch\u1ee5p \u0111\u01b0\u1ee3c trong b\u00e0o t\u1ebf bi\u1ec7t h\u00f3a c\u1ee7a \u1ed1ng gan t\u1ee5y t\u00f4m<\/span><\/em><\/strong><\/p>\n<p style=\"margin-bottom: .0001pt; text-align: justify; text-justify: inter-ideograph; line-height: normal;\"><em><span style=\"font-family: times new roman,serif;\">A: H\u00ecnh ch\u1ee5p vi m\u00f4 c\u1ee7a c\u00e1c t\u1ea1p ch\u1ea5t \u00e1i ki\u1ec1m WZV8 trong c\u00e1c t\u1ebf b\u00e0o bi\u1ec7t h\u00f3a (t\u1ebf b\u00e0o R) c\u1ee7a gan t\u1ee5y t\u00f4m<\/span><\/em><\/p>\n<p style=\"margin-bottom: .0001pt; text-align: justify; text-justify: inter-ideograph; line-height: normal;\"><em><span style=\"font-family: times new roman,serif;\">B: H\u00ecnh \u1ea3nh ch\u1ee5p c\u00e1c ph\u1ea7n b\u00e1n m\u1ecfng c\u1ee7a m\u00f4 h\u1ec7 gan tu\u1ef5 \u1edf t\u00f4m cho th\u1ea5y s\u1ef1 thay \u0111\u1ed5i trong th\u1ec3 v\u00f9i WZV8 (m\u00e0u xanh \u0111\u1eadm v\u00e0 h\u00ecnh tr\u00f2n) trong t\u1ebf b\u00e0o ph\u00f4i h\u1ec7 gan t\u1ee5y t\u00f4m. M\u1ed9t s\u1ed1 hi\u1ec3n th\u1ecb m\u1ed9t v\u1ec7 tinh nh\u1ecf h\u01a1n, li\u1ec1n k\u1ec1 th\u1ec3 v\u00f9i ho\u1eb7c c\u00e1c kh\u00f4ng b\u00e0o kh\u00e1c.<\/span><\/em><\/p>\n<ul>\n<li style=\"margin-bottom: 6pt; text-align: justify;\"><strong><em><u><span style=\"font-family: times new roman,serif;\"><span style=\"font-size: 13.0pt;\">Ph\u00e1t hi\u1ec7n WZV8 b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p x\u00e9t nghi\u1ec7m ph\u00e2n t\u1eed<\/span><\/span><\/u><\/em><\/strong><\/li>\n<\/ul>\n<p style=\"margin-bottom: 6.0pt; text-align: justify; text-justify: inter-ideograph; text-indent: 36.0pt;\"><span style=\"font-family: times new roman,serif;\"><span style=\"font-size: 13.0pt;\">C\u00e1c nh\u00e0 nghi\u00ean c\u1ee9u Th\u00e1i Lan \u0111\u00e3 s\u1eed d\u1ee5ng th\u00e0nh c\u00f4ng ph\u01b0\u01a1ng ph\u00e1p RT-PCR \u0111\u1ec3 s\u00e0ng l\u1ecdc t\u00f4m qua c\u00e1c m\u1eabu RNA t\u1eeb c\u00e1c kh\u00e1ch h\u00e0ng to\u00e0n c\u1ea7u. \u0110i\u1ec1u n\u00e0y s\u1ebd cung c\u1ea5p ngu\u1ed3n plasmid v\u0129nh vi\u1ec5n \u0111\u1ec3 s\u1eed d\u1ee5ng l\u00e0m \u0111\u1ed1i ch\u1ee9ng d\u01b0\u01a1ng t\u00ednh trong c\u00e1c x\u00e9t nghi\u1ec7m RT-PCR v\u00e0 l\u00e0m m\u1ee5c ti\u00eau \u0111\u1ec3 t\u1ea1o ra m\u1ed9t \u0111\u1ea7u d\u00f2 c\u1ee5 th\u1ec3 DNA c\u1ee7a WZV8 b\u1eb1ng PCR. Tr\u00ecnh t\u1ef1 \u0111o\u1ea1n m\u1ed3i \u0111\u01b0\u1ee3c th\u1ec3 hi\u1ec7n trong b\u1ea3ng d\u01b0\u1edbi \u0111\u00e2y.\u00a0<\/span><\/span><\/p>\n<img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-3537 size-full\" src=\"http:\/\/sunjin.vn\/wp-content\/uploads\/2023\/05\/E02_5.png\" alt=\"\" width=\"837\" height=\"191\" \/>\n<p style=\"margin-bottom: 6.0pt; text-align: center;\" align=\"center\"><strong><em><span style=\"font-family: times new roman,serif;\">B\u1ea3ng: C\u00e1c primer \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng cho khu\u1ebfch \u0111\u1ea1i l\u1ed3ng gh\u00e9p c\u1ee7a RT-PCR c\u1ee7a nghi\u00ean c\u1ee9u n\u00e0y<\/span><\/em><\/strong><\/p>\n<p style=\"margin-bottom: 6.0pt; text-align: justify; text-justify: inter-ideograph; text-indent: 36.0pt;\"><span style=\"font-family: times new roman,serif;\"><span style=\"font-size: 13.0pt;\">Ph\u1ea3n \u1ee9ng RT-PCR b\u01b0\u1edbc \u0111\u1ea7u \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n trong 12,5 \u00b5l h\u1ed7n h\u1ee3p g\u1ed3m 1X Ph\u1ea3n \u1ee9ng Tr\u1ed9n (Invitrogen, Hoa K\u1ef3); 0,4 \u00b5M m\u1ed7i \u0111o\u1ea1n m\u1ed3i 482F v\u00e0 482R; 0,5 \u00b5l SuperScript III RT\/Platinum Taq Mix (Invitrogen, Hoa K\u1ef3) v\u00e0 100 ng khu\u00f4n m\u1eabu RNA. Giao th\u1ee9c RT-PCR b\u1eaft \u0111\u1ea7u v\u1edbi 50\u00b0C trong 30 ph\u00fat, sau \u0111\u00f3 l\u00e0 94\u00b0C trong 2 ph\u00fat v\u00e0 sau \u0111\u00f3 l\u00e0 35 chu k\u1ef3 94\u00b0C trong 30 gi\u00e2y, 60\u00b0C trong 30 gi\u00e2y v\u00e0 68\u00b0C trong 45 gi\u00e2y, c\u1ed9ng v\u1edbi th\u1eddi gian k\u00e9o d\u00e0i cu\u1ed1i c\u00f9ng \u1edf 68\u00b0C trong 5 ph\u00fat.<\/span><\/span><\/p>\n<img loading=\"lazy\" decoding=\"async\" class=\"wp-image-3536 size-full aligncenter\" src=\"http:\/\/sunjin.vn\/wp-content\/uploads\/2023\/05\/E02_6.png\" alt=\"\" width=\"586\" height=\"314\" \/>\n<p style=\"margin-bottom: 6.0pt; text-align: center;\" align=\"center\"><strong><em><span style=\"font-family: times new roman,serif;\">Khu\u1ebfch \u0111\u1ea1i DNA b\u1eb1ng c\u00e1ch pha lo\u00e3ng n\u1ed1i ti\u1ebfp s\u1ea3n ph\u1ea9m PCR tinh khi\u1ebft c\u1ee7a WZV8 t\u1ea1i n\u1ed3ng \u0111\u1ed9 t\u1eeb 2 \u0111\u1ebfn 2\u00d710<sup>5<\/sup> b\u1ea3n sao\/ph\u1ea3n \u1ee9ng.<\/span><\/em><\/strong><\/p>\n<p style=\"margin-bottom: 6.0pt; text-align: justify; text-justify: inter-ideograph; text-indent: 36.0pt;\"><span style=\"font-family: times new roman,serif;\"><span style=\"font-size: 13.0pt;\">\u0110\u1ed1i v\u1edbi b\u01b0\u1edbc PCR, h\u1ed7n h\u1ee3p 12,5 \u00b5l ch\u1ee9a 1X OneTaq Hot Start Master Mix (NEB, Hoa K\u1ef3); 0,2 \u00b5M c\u1ee7a m\u1ed7i \u0111o\u1ea1n m\u1ed3i 168F v\u00e0 168R; l \u00b5l dung d\u1ecbch s\u1ea3n ph\u1ea9m t\u1eeb b\u01b0\u1edbc RT-PCR \u0111\u1ea7u ti\u00ean. Giao th\u1ee9c PCR l\u1ed3ng nhau l\u00e0 94\u00b0C trong 5 ph\u00fat, sau \u0111\u00f3 l\u00e0 25 chu k\u1ef3 94\u00baC trong 30 gi\u00e2y, 60\u00baC trong 30 gi\u00e2y v\u00e0 72\u00baC trong 30 gi\u00e2y c\u1ed9ng v\u1edbi ph\u1ea7n m\u1edf r\u1ed9ng cu\u1ed1i c\u00f9ng trong 5 ph\u00fat \u1edf 72\u00baC. C\u00e1c amplicon thu \u0111\u01b0\u1ee3c l\u1ea7n l\u01b0\u1ee3t l\u00e0 482 bp v\u00e0 168 bp,<\/span><\/span><\/p>\n<p style=\"margin-bottom: 6.0pt; text-align: justify; text-justify: inter-ideograph; text-indent: 36.0pt;\"><span style=\"font-family: times new roman,serif;\"><span style=\"font-size: 13.0pt;\">V\u1edbi c\u00e1c nghi\u00ean c\u1ee9u h\u1ed7 tr\u1ee3 cho vi\u1ec7c ph\u00e1t hi\u1ec7n virus WZV8 t\u1eeb c\u00e1c nh\u00e0 khoa h\u1ecdc Th\u00e1i Lan, \u0111i\u1ec1u n\u00e0y cho ph\u00e9p ch\u00fang ta ti\u1ebfp c\u1eadn khoa h\u1ecdc nhanh ch\u00f3ng, \u0111\u1ea9y nhanh s\u1ef1 h\u1ee3p t\u00e1c qu\u1ed1c t\u1ebf nh\u1eb1m \u0111\u00e1nh gi\u00e1 c\u00e1c m\u1ed1i \u0111e d\u1ecda v\u00e0 h\u1ea1n ch\u1ebf s\u1ef1 l\u00e2y lan c\u1ee7a m\u1ea7m b\u1ec7nh WZV8 ti\u1ec1m t\u00e0ng.<\/span><\/span><\/p>\n<p style=\"margin-bottom: 6.0pt; text-align: justify; text-justify: inter-ideograph; text-indent: 36.0pt;\"><strong><span style=\"font-family: times new roman,serif;\"><span style=\"font-size: 13.0pt;\">L\u01b0\u1ee3t d\u1ecbch t\u1eeb b\u00e0i <em>\u201cWenzhou virus 8 (WZV8) diagnosis by unique inclusions in shrimp hepatopancreatic E-cells and a molecular detection method\u201d.<\/em><\/span><\/span><\/strong><\/p>\n<p style=\"text-align: right;\" align=\"right\"><strong><em><span style=\"font-family: times new roman,serif;\"><span style=\"font-size: 13.0pt;\">Ngu\u1ed3n: Trung t\u00e2m Nghi\u00ean c\u1ee9u \u0110\u1ed5i m\u1edbi Th\u1ee7y s\u1ea3n &#8211; AIC<\/span><\/span><\/em><\/strong><\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Sunjin \u0111\u1ea7u t\u01b0 x\u00e2y d\u1ef1ng nh\u00e0 m\u00e1y Th\u1ee7y s\u1ea3n t\u1ea1i t\u1ec9nh H\u1eadu Giang &#038; chuy\u00ean m\u1ee5c k\u1ef9 thu\u1eadt &#8211; T\u00ecm hi\u1ec3u v\u1ec1 Wenzhou virus 8 (WZV8) tr\u00ean t\u00f4m v\u00e0 ph\u01b0\u01a1ng ph\u00e1p ph\u00e1t hi\u1ec7n<\/p>\n","protected":false},"author":1,"featured_media":5838,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"footnotes":""},"categories":[208],"tags":[],"class_list":["post-6400","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-khong-phan-loai-en"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v20.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Tin t\u1ee9c Th\u1ee7y s\u1ea3n s\u1ed1 E02- 02\/2023 - Sunjinvn<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"http:\/\/sunjin.vn\/en\/tin-tuc-thuy-san-so-e02-022023\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Tin t\u1ee9c Th\u1ee7y s\u1ea3n s\u1ed1 E02- 02\/2023 - Sunjinvn\" \/>\n<meta property=\"og:description\" content=\"Sunjin \u0111\u1ea7u t\u01b0 x\u00e2y d\u1ef1ng nh\u00e0 m\u00e1y Th\u1ee7y s\u1ea3n t\u1ea1i t\u1ec9nh H\u1eadu Giang &amp; 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